Perfect PE Antibody for Looking at RANKL Receptor Expression on BMMs - Unattributed Review*

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

BioLegend

Product Name:

Anti-mouse RANKL receptor/ TRANCE

Catalog Number:

510005

In vitro cultured Bone marrow cells stimulated with M-CSF and RANKL and RANKL receptor expression with CSF was checked on BMMs.

*Reviews submitted to our site where we are unable to verify the identity of the author are considered “unattributed reviews".

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Bone marrow

Primary Incubation

1:400 of anti-mouse PE CD254

Blocking Agent

FBS/PBS

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

NA

Results Summary

Specific staining results were obtained and differences could be seen between WT and KO mice that we are using in our laboratory.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Good color conjugation as the receptor expression is very low. PE works nicely enough when used at the right dilution

The Bad

None

The Bottom Line

Specific antibody gives high signal even with low receptor expression of RANK

Share your experience with other scientists. Write a Review! »

Join the discussion