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PE Anti-human CD38 Antibody
303506
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CD38 is considered a marker of immune activation expressed in T, B and NK cells. The role of CD38 in CD8+ T cell function against viral infection has been recently in focus and co-expression of PD-1 has been termed as a condition which favors progression of disease. Thus, CD8+ T cells with increased co-expression of CD38 and PD-1 have been termed progressor CD8 T cells and the ones with lower levels of co-expression as controller T cells. It should be kept in mind that this is a relative phenomenon and there will certainly be some percentage of co-expression in CD8+ T cells for these two markers at a given time point.
Flow cytometry
PBMC, lymphocytes
PBMC's form human patients infected with virus were thawed. Cells were washed twice with PBS and stained for the following antibodies- CD4, CD8, CD38, PD-1. Staining was done by diluting the antibodies 1:50 and preparing a cocktail for all the samples except the isotype, unstained, and single color controls. Cells were incubated with the antibody cocktail for 30 mins at 4 degree C/ over ice. Cells were washed twice with PBS and re-suspended in FACS buffer for flow cytometric analysis.
Preparation of cocktail of antibodies is important to standardize results. Prepare cocktails for n+2 number of samples.
Expression of CD38 and PD-1 were clearly visible in CD8+ T cells (gated on CD8+ population). The percentage of co-expression of CD38 and PD-1 was indicative of progressor vs. controller CD8 T cells.
None
Good florescence, easily distinguishable.
Easy, reliable and repeatable method of surface staining of CD8+ T cells to identify distinct population co-expressing CD38 and PD-1
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