Fast and Easy Plasmid DNA Purification Kit

Carnegie Institution for Science
Embryology
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Overall

Quality of Results

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Company:

Zymo Research

Product Name:

ZymoPure Midi Prep Kit

Catalog Number:

D4200

We used to use Qiagen HiSpeed Plasmid Midi Kit for Plasmid purification. It took a long time for the whole process. Zymoreseach's ZymoPure kit really save us a lot of time and energy for this tedious process. It always give us high yield and quality DNA.

Experimental Design and Results Summary

Application

Plasmid DNA isolation

Starting Material

50mL DH5a bacteria

Protocol Overview

1. Centrifuge to precipitate bacteria. 2. Simply follow the instructions of the Kit: lysis, neutralization and pass column. 3. After washing, elute Plasmid DNA.

Tips

Using a vacuum will save a lot of time when processing many samples together

Results Summary

I always get very good yields of plasmid DNA and purity with this kit. The 260/280 is higher than 1.80

Additional Notes

I really like the colored buffer system, which makes it easy to hand each step.

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Summary

The Good

Fast, simple and cheap plasmid midi prep Kit

The Bad

None

The Bottom Line

A much better Plasmid Midi Prep kit than Qiagen: Fast, simple, reliable and cheap.

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