Survivin Expression in Mouse T Cells

Immunology
Northwestern University
Grad Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

Survivin (71G4B7) Rabbit mAb

Catalog Number:

2808

The aim of the research was to determine the levels of Survivin in unstimulated and stimulated mouse T cells. T cells were stimulated for 24h with anti-CD3/28 Survivin levels were measured by Western blot.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse T cells

Primary Incubation

1:4000, overnight, rocking, 4 degrees

Blocking Agent

10% Milk

Secondary Incubation

1:10,000 in 5% milk, 30 min

Tertiary Incubation

NA

Detection

Chemiluminescence

Results Summary

Clean antibody with low background and sharp bands.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Easy to optimize, low background

The Bad

Nothing negative to comment

The Bottom Line

Great antibody for detecting mouse Survivin.

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