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Anti-mouse IgG1 antibody
406604
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To test antibody responses of TRPM2 -/- mice, wild-type (Trpm2+/+) or Trpm2−/− mice were subcutaneously immunized on day 0 and day 14 with 40 μg/mouse ovalbumin (OVA) alone or the same amount of OVA encapsulated within DOTAP liposomes. On day 24, the mice were sacrificed, and sera were collected and analyzed for OVA-specific IgG1, IgG2b by ELISA. We found IgG1 level was reduced in OVA-DOTAP immunized mice.
ELISA
Serially diluted mouse sera was added in microplate precoated with 5ug/ml OVA and incubated for 1 hr at 37 degrees
Biotin labeled anti-mouse IgG1 antibody (0.5 ug/ml) was added and incubated for 1 hr at 37 degrees
5% nonfat milk in PBST was used for plate blocking after OVA protein coating
HPR-streptavidin was added (1:10,000) and incubated for 1 hr at 37 degrees
NA
TMB was used for signal development
We found that the level of ovalbumin (OVA)-specific IgG1 was significantly reduced in Trpm2−/− mice after immunization with OVA-encapsulated DOTAP liposomes. The antibody is sensitive and specific for ELISA.
None
This antibody is sensitive and specific for detecting antigen specific IgG1 antibody isotype.
The antibody is able to specifically detect mouse IgG1 isotype antibody by ELISA. We do not see much non-specific reactions.