pSTAT3 in Th17 Cells

November 10, 2015

Immunology
Northwestern University
Grad Student

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Cell Signaling Technology

Product Name:

Phospho-Stat3 (Tyr705) Antibody

Catalog Number:

9131

Info: View Product View Product Specs

Browse Similar Products: pSTAT3 Antibodies

The aim of my research was to determine the time course of IL-6-induced STAT3 phosphorylation in mouse Th17 cells.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse Th17 cells

Primary Incubation

1:1000, overnight, rocking, 4 degrees

Blocking Agent

10% Milk

Secondary Incubation

1:10,000 in 5% milk, 30 min

Tertiary Incubation

NA

Detection

Chemiluminescence

Results Summary

Low background. Clear and sharp bands.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Easy to optimize

The Bad

Nothing negative to comment

The Bottom Line

Great antibody for determining the phosphorylation of STAT3 at Tyr705.

Share your experience with other scientists. Write a Review! »

Join the discussion