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FITC-aMouse Ly-6G
127605
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To analyze neutrophil recruitment, mice were pretreated with PBS or a drug (LF fraction 10). 48 hours later, mice were injected with LPS via i.p. and peritoneal cells were collected 4 hours later. Cells were blocked with Fc blocking antibody and then stained with FITC-anti mouse Ly-6G. Cells were analyzed by FACS. We found that treatment inhibited LPS induced neutrophil recruitment in the peritoneal cavity.
Flow Cytometry
Mouse freshly isolated peritoneal cells
Anti-mouse CD16/32 Antibody
5% FBS in PBS
FITC-aM Ly-6G
NA
Flow cytometry Canto II
The drug treatment inhibited LPS induced neutrophil recruitment in the peritoneal cavity. While PBS treated mice showed increased neutrophil activity. The antibody is specific and sensitive for staining the neutrophil marker.
Antibody incubation on ice for 15-30 min is enough
The antibody is specific and sensitive in one step staining.
None
This antibody is good for FACS experiments for staining a neutrophil population. I have not yet combined it with other antibodies to costain cells.