Cell Signaling Technology
p38 MAPK Antibody #9212
9212
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Cells were treated and proteins were extracted to check the normal p38MAPK levels by western blotting. Equal amounts of proteins were resolved in 10% SDS-PAGE and immuoblotted with p38MAPK antibody from Cell Signaling. Selection of antibody was based on merits like endogenous detection in mammalian cells and on its specificity.
Immunoblotting
Whole cell protein lysate
1:1000 in skimmed milk prepared in 1X TBST, 4C overnight
Skim milk (5%) prepared in TBST for 2hr
Goat anti-rabbit IgG-HRP, 1:4000 dilution in skimmed milk, 1h at room temperature
None
ECL
p38MAPK antibody from Cell Signaling has showed excellent specific bands. This antibody is good for immunoblotting.
Highly specific
Got some non-specific bands
Best antibody from Cell Signaling