B220 Pacific Blue Antibody Review

November 09, 2015

Microbiology
Medical College of Wisconsin
Research Technologist

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
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Company:

BioLegend

Product Name:

B220 Pacific Blue

Catalog Number:

103230

Info: View Product View Product Specs

Browse Similar Products: CD45/B220 Antibodies

Mice are infected with virus, then splenocytes or PECs are collected, RBC lysed, blocked, and stained.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Splenocytes

Primary Incubation

1:100, 15 minutes

Blocking Agent

Fc Block

Secondary Incubation

1:500, 15 minutes

Tertiary Incubation

NA

Detection

Flow Cytometry LSRII

Results Summary

Reliable antibody for staining of B Cells, not as robust as others but may depend on experimental variables.

Additional Notes

None

Related Categories

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Summary

The Good

Reliable

The Bad

Not very robust

The Bottom Line

Would recommend this antibody, we use it regularly for many experiments to detect B cells.

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