Mouse IL25 ELISA Kit

Harvard Medical School
Medicine
Post-doctoral fellow

Overall

Quality of Results

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Company:

eBioscience

Product Name:

Mouse IL-17E (IL-25) ELISA Ready-SET-Go

Catalog Number:

88-7002

I study release of various cytokines by immune cells in response to antigens in vitro as well as in vivo.

Experimental Design and Results Summary

Application

Cytokine ELISA

Starting Material

Cell culture supernatants

Protocol Overview

Culture supernatants were incubated overnight at 4 degrees, followed by washings with wash buffer and detection antibody. ELISA was revealed by avidin-HRP and TMB solution.

Tips

Standardization is necessary to work out the right dilution for supernatants

Results Summary

Cytokine release was detected in response to various antigens

Additional Notes

None

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Summary

The Good

Good ELISA kit for IL25 detection

The Bad

Requires overnight plate coating step

The Bottom Line

Good and cost-effective ELISA assay

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