Sigma-Aldrich
IPTG
I6758
I studied protein-protein interaction using bacteriomatch two and three hybrid system in X-gal indicator plates and used IPTG and Arabinose and inducers for gene expression from different plasmids.
Protein-protein interaction
X gal plates, and BacterioMatch II Two-Hybrid System
Third plasmid under arabinose induction was transformed to competent cells containing two plasmids under IPTG induction (containing two interacting proteins)
None
Found one peptide that could disrupt ESAT6-CFP10 interaction in a bacterial three hybrid system
Good for biochemical and bacteriological assays