Good Anti-FoxP3 Antibody for Nuclear Staining

Medicine
Harvard Medical School
Post-doctoral fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

eBioscience

Product Name:

Anti-Foxp3 PE

Catalog Number:

12-4774-42

Info: View Product View Product Specs

Browse Similar Products: FoxP3 Antibodies

I studied immune response in mice against mycobacterium tuberculosis H37Rv and clearance of the pathogen from mouse systems using anti-mycobacterial ESAT6 binding peptides.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mice splenocytes and lung T-cells

Primary Incubation

1:50 dilution, after blocking with 2% BSA and Fc block for 1 hour on ice

Blocking Agent

Blocking with 2% BSA and Fc block

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Flow Cytometry

Results Summary

The ESAT6 binding peptides enhanced the immune response against mycobacterium tuberculosis and resulted in faster clearance of the bacteria from the lungs and spleen. ESAT6 binding peptide also led to a decrease in regulatory T-cells.

Additional Notes

None

Related Categories

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Summary

The Good

Good flow cytometry antibody, gives nice signal

The Bad

None

The Bottom Line

Good antibody for nuclear staining of FoxP3, works nicely for studying regulatory T cells

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