GCLM Antibody NBP1-33405 from Novus Biologicals

November 09, 2015

Institute of Genetic Medicine
Johns Hopkins University
Postdoctoral Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

Novus Biologicals

Product Name:

GCLM Antibody

Catalog Number:

NBP1-33405

This antibody is used to detect the expression of GCLM in different breast cancer cell lines.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human breast cancer cell line whole cell lysate

Primary Incubation

1:2000, overnight at 4 degree

Blocking Agent

5% milk

Secondary Incubation

1:2000, 1 hour at RT

Tertiary Incubation

N/A

Detection

ECL Prime

Results Summary

This antibody detect specific GCLM band with some non-specific bands by western blot.

Additional Notes

Related Categories

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Summary

The Good

detect specific band and the signal is strong

The Bad

there are some non-specific bands

The Bottom Line

This antibody can easily detect GCLM expression by western blot.

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