Good ELISA Kit for Detecting Human MCP-1 Protein

November 06, 2015

RUSH UNIVERSITY MEDICAL CENTER
ORTHOPAEDIC SURGERY
POSTDOC

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Company:

Novex, Thermo Fisher Scientific

Product Name:

Human MCP-1 antibody pair

Catalog Number:

CHC1013

This kit includes coating antibody, detection antibody, standards and streptavidin-HRP reagents. Need to order buffers and solution seperately. Need to coat the plate by yourself. One kit is good for 10 96-well plates.

Experimental Design and Results Summary

Application

ELISA

Starting Material

None

Protocol Overview

Coat the plate overnight with suggested concentration and wash briefly. Then add in sample and detection antibody and wash. Add in Streptavidin-HRP and wash. Add in substrate solution and finally stop solution.

Tips

Coating buffer needs the basic coating buffer.

Results Summary

The antibody and reagents provided in the kit works well in detecting the MCP-1 protein concentration in the cell culture media.

Additional Notes

None

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Summary

The Good

One kit is good for ten plates and could do a lot of samples.

The Bad

Need to buy suggested buffers and solution seperately.

The Bottom Line

This kit is good and works well in detecting human MCP-1 protein concentration in cell culture media.

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