Good ELISA Kit for Human IL-6 Protein Detection

RUSH UNIVERSITY MEDICAL CENTER
ORTHOPAEDIC SURGERY
POSTDOC

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Invitrogen, Thermo Fisher Scientific

Product Name:

Human IL-6 Cytoset

Catalog Number:

CHC1263

This kit includes standards, coating antibody, detection antibody and Streptavidin-HRP reagents. Need to coat the plate by yourself. Works well in detecting human cultured cell media IL-6.

Experimental Design and Results Summary

Application

ELISA

Starting Material

Human cell culture media

Protocol Overview

Coat the plate then add in sample and detection antibody and wash. Add in Streptavidin-HRP and wash. Add in substrate solution and finally stop solution.

Tips

Double the coating antibody concentration if necessary.

Results Summary

This kit generally works well in the human annulus fibrosus cell culture media.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

One kit is good for ten plates and could work for a lot of samples.

The Bad

Need to order buffers and solutions seperately.

The Bottom Line

This is a good kit and it works well for detecting the human cell culture media IL-6 concentration.

Join the discussion