BD Biosciences
PE Rat Anti-Mouse CD16/CD32
561727
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I wanted to look at whether expression of CD16/32 on myeloid cells in the small intestine appeared to differ in a healthy vs diseased mouse.
Immunofluorescence
Pre-fixed frozen mouse small intestine tissue section
1:100 in 1% BSA in PBS overnight at 4 degrees
5% goat serum in 1% BSA in PBS
Goat anti-rat AlexaFluor594 was used at 1:200 in PBS and was incubated on the section for 1 hour at room temperature in the dark
NA
Zeiss LSM 700 confocal
CD16/32 staining was clearly visualized in myeloid-like cells in the small intestine of diseased mice, and shows the proper membrane-receptor staining pattern.
The green stain here is CX3CR1-GFP
Good staining of CD16/32 by immunofluorescence
None to mention
A great antibody for staining CD16/32 via immunofluorescence