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Brilliant violet 421 anti-mouse CD25
102043
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CD25 or IL-2 receptor alpha is only expressed on Treg cells and transiently expressed on activated T cells. I have never performed CD25 staining on the brilliant violet channel; so wanted to test this antibody as other channels were occupied. The stain using BV421 was as good as other bright fluorophores like PE, FITC etc. as observed in my previous experiments.
Flow Cytometry
Lymphocytes
1:200 in PBS for 30 minutes
FcR block
NA
Flow Cytometry - BV421 channel
There is a clear CD25 positive population on ex vivo isolated lymphocytes from a mouse spleen. The frequency of positives is physiological. The populations are not as distinct as observed with other bright fluorophores.
None
It works well on this channel
Populations were not distinctly separated
It will work if you have exhausted other channels.