L (+) Arabinose for Bacteriological and Biochemical Purposes

Harvard Medical School
Medicine
Post-doctoral fellow

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Company:

Himedia Labs

Product Name:

L (+) Arabinose

Catalog Number:

GRM037

I studied disruption of ESAT6 and its natural binder CFP10 by another novel peptide, using a bacterial three hybrid assay.

Experimental Design and Results Summary

Application

Biochemical assay (Liquid beta-galactosidase assay)

Starting Material

Bacterial cell pellets from three hybrid assays

Protocol Overview

Disruption of the ESAT-6:CFP10 interaction by HCL2, using an Arabinose gradient liquid β-Galactosidase assay. Assay was carried out by varying L-arabinose concentrations ranging from 0 to 1% to increase the amount of disruptor peptides expression.

Tips

None

Results Summary

HCL2 efficiently disrupts potent ESAT6-CFP10 interaction.

Additional Notes

None

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Summary

The Good

Good for biochemical assays

The Bad

None

The Bottom Line

Good for arabinose inducible plasmids

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