Nice RT-PCR Kit

University of Illinois, Chicago
Department of Microbiology and Immunology
PostDoctoral Research Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

New England Biolabs. Inc

Product Name:

OneTaq® RT-PCR Kit

Catalog Number:

E5310S

I amplified GAPDH as a loading control for 293T cells using NEB's one tag RT-PCR kit. It works pretty well for mRNA analysis.

Experimental Design and Results Summary

Application

Reverse transcriptase PCR

Starting Material

cDNA

Protocol Overview

All the ingredients provided in the kit were mixed together with GAPDH primers and cDNA of 293T cells. PCR amplification was carried out using Bio-Rad's PCR.

Tips

Minimize pipetting errors by using different tips for each pipetting.

Results Summary

This kit is highly efficient and gives clean results. only specifc bands were obtained with no background.

Additional Notes

None

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Summary

The Good

Quick and reliable kit

The Bad

None

The Bottom Line

Wonderful kit for RT-PCR.

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