Best Kit for cDNA Synthesis

University of Illinois, Chicago
Department of Microbiology and Immunology
PostDoctoral Research Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

Bio-Rad

Product Name:

iScript™ cDNA Synthesis Kit #1708891

Catalog Number:

1708891

Info: Supplier Page 

I used Bio-Rad's cDNA synthesis kit for Reverse-transcriptase PCR reaction. I prepared 20 microlitres of cDNA for each sample and then amplified it using GAPDH primers. All the cDNAs prepared are of optimum quality and this kit works for all my RT-PCR and qPCR assays.

Experimental Design and Results Summary

Application

Reverse transcriptase PCR

Starting Material

Total RNA

Protocol Overview

I used 1 microgram of total RNA for each sample and prepared 20 microlitres of cDNA. 1 microlitres of cDNA was used to further amplify GAPDH.

Tips

None

Results Summary

All cDNAs were of optimal quality.

Additional Notes

None

Related Categories

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Summary

The Good

Great kit, easy to use, single step cDNA preparation and has all the reagents with it

The Bad

None

The Bottom Line

In conclusion. It's one of the best kits for cDNA synthesis

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