Cell Signaling Technology
Akt antibody
9272
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I check total endogenous AKt expression in two cell lines: 293T and BT474. The antibody worked fine but gave a non-specific band at low molecular weight.
Western Blot
Whole cell lysate
1:1000 diluted in 5% non fat dry milk in TBST, incubated at RT for an hour
5% non fat dry milk in TBST
Anti-rabbit secondary antibody at 1:3000 dilution in blocking solution, incubated at room temperature for an hour
NA
ECL
This antibody gives no background and gives sharp bands.
None
Optimized manufacturer's protocol
One non-specific band of low molecular weight
In total, it's a good antibody