Excellent ECL Substrate

University of Illinois, Chicago
Department of Microbiology and Immunology
PostDoctoral Research Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

Thermo Fisher Scientific

Product Name:

Pierce™ ECL Western Blotting Substrate

Catalog Number:

32106

I use this kit for developing blots by ECL. It's very convenient to use, gives no background, and is highly sensitive. I tried some other brands for ECL substrates such as Amersham, but they were not up to the mark in performance in terms of specifciity and sensitivity. This one is my favorite.

Experimental Design and Results Summary

Application

Western Blot

Starting Material

Membrane probed with specific antibodies

Protocol Overview

We have two bottles inside each kit. Mix Detection Reagents 1 and 2 at a 1:1 ratio and add it to the blot. Incubate blot for 1 minute and remove excess of the reagent. Develop the blot by exposing. to X-Ray films

Tips

Use it in the dark

Results Summary

Excellent ECL substrate with high performance.

Additional Notes

None

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Summary

The Good

Highly specific and sensitive substrate

The Bad

None

The Bottom Line

Perfect substrate for western blotting

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