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Brilliant violet 410 anti-mouse CD19
115549
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I used this antibody for staining B cells within lymphocytes. Compared to other fluorophores (BV510 etc.), this antibody showed much brighter expression and higher fluorescence in the positive population. The background was minimal.
Flow Cytometry
Lymphocytes
1:200 in PBS for 30 minutes
FcR block
NA
Flow Cytometry - BV421 channel
As seen in the histograms, a bright and distinct population of CD19+ B cells is seen after staining. The background seems minimal.
None
Bright stain and easy to use
Sometimes difficult to compensate with other bright fluorophores
Great choice for staining B cell lineage on the violet laser