Cell Signaling Technology
ATF-2 (20F1) Rabbit mAb
9226S
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Our aim was to identify mechanisms underlying ATF2 subcellular localization since nuclear localization is associated with poor prognosis in melanoma.
Immunofluorescence
Melanoma and SCC cell lines
1:100, overnight @ 4C
0.1% TX-100/0.1% BSA in PBS
Goat anti-rabbit Alexa-568, 1:10000, 1h RT
NA
Fluorescence Microscope
Nuclear, cytoplasmic or mitochondrial staining depending on cell line and culture condition. SCC cells in steady state show nuclear staining while after genotoxic stress, ATF2 staining is both mitochondrial and nuclear.
None
High Specificity - low background
Overnight incubation, need to use low dilution
Compared to the other ATF2 antibodies available, this antibody gives the most specific staining with low background.