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PerCP-Cy5.5 anti-mouse CD3
100218
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PERCP-Cy5.5 is not the best channel, but was the only one available in my staining panel. So, I decided to use it for a lineage marker like anti-CD3. Usually, the expression is high enough to get a bright 2-3 log high signal. However, the signal with this antibody was relatively low; to the point where some intermediate cells could not be distinguished as either positives or negatives.
Flow Cytometry
Lymphocytes
1:200 in PBS for 30 minutes
FcR block
NA
Flow Cytometry - PerCP-Cy5.5 channel
There is a positive population; however, the fluorescence was quite low. Not recommended for sorting type applications. Some folks suggested using intra-cellular staining to brighten the signal.
None
Easy to use
Very weak fluorescence
Good antibody as an option for staining but not the best fluorophore.