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Brilliant violet anti-mouse CD4
100548
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CD4 is expressed on MHC-II restricted T cells within lymphocytes. Since the population is well-characterized, I wanted to test if it can be detected using a dim fluorophore like BV605. Even though the staining is dim, the positive populations looks quite distinct with minimal background.
Flow Cytometry
Lymphocytes
1:200 in PBS for 30 minutes
FcR block
NA
Flow Cytometry - BV605 channel
The staining looks dim, but the population is quite distinct from the negative control.
None
Distinct staining
Dim fluorescence
It's a great choice when bright fluorophores are not available in your staining panel.