Life Technologies
LIVE/DEAD Fixable Near-IR Dead Cell Stain Kit
L10119
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This kit is used with a red laser flow cytometer. The benefits of using this kit are that you can fix cells after staining (unlike most other dyes) and the fluorescence is quite robust.
Flow Cytometry
Lymphocytes
1:1000 in PBS for 30 minutes
FcR block
NA
Near IR fluorescence channel on flow cytometry
The stain shows a bright signal, even after the cells were fixed and stored for several days before analysis. The separation between live and dead cells is quite evident.
None
Bright stain
Fluorecence bleeds into other channels
Great antibody for larger preps or intracellular stains where samples need to be fixed.