Good IgG Antibody for ELISA

University of Illinois, Chicago
Department of Microbiology and Immunology
PostDoctoral Research Fellow

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Company:

Jackson Immunoresearch Laboratories

Product Name:

Horsereddish-peroxidase conjugated anti-human IgG

Catalog Number:

109-035-003

I checked circulating anti-AKAP4 antibodies in human serum by standard ELISA. This secondary antibody works pretty well without non-specififc background.

Experimental Design and Results Summary

Application

ELISA

Starting Material

Human serum samples

Protocol Overview

To check antibodies in human sera, anti-human IgG was diluted at 1:1000 and incubated for an hour at RT

Tips

Keep the plates on a shaker while incubating

Results Summary

Circulating antibodies were detected in different clinical groups.

Additional Notes

None

Image Gallery

Summary

The Good

Specificity

The Bad

No detetction at higher dilutions

The Bottom Line

Good antibody for ELISA purposes

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