Good Flow Cytometry Antibody, Gives Nice Signal

November 02, 2015

Medicine
Harvard Medical School
Post-doctoral fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

PE anti-mouse CD69 Antibody

Catalog Number:

104508

Info: View Product View Product Specs

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I studied immune response in mice against mycobacterium tuberculosis H37Rv and clearance of the pathogen from the mouse system using anti-mycobacterial ESAT6 binding peptides.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse lung lymphocytes

Primary Incubation

1:100 dilution, after blocking with 2% BSA and Fc block for 1 hour on ice

Blocking Agent

Blocking with 2% BSA and Fc block

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow Cytometry

Results Summary

Good antibody for surface staining of CD69

Additional Notes

None

Related Categories

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Summary

The Good

Good flow cytometry antibody, gives nice signal

The Bad

None

The Bottom Line

The antibody is good for surface staining for CD69. And useful for studying activation of T cells.

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