Labeling of Human PBMCs with CD62L BV421 Antibody

October 29, 2015

Anaesthesiology
LMU Munich
Researcher

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Quality of Results

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Company:

BioLegend

Product Name:

CD62L Antibody

Catalog Number:

304827

Info: View Product View Product Specs

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This CD62L antibody is included in a flow cytometry panel in order to identify L-Selectin expression on human PBMCs. Because multiple receptors needed to be stained, we used the BV421 conjugation of this antibody.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human PBMCs

Primary Incubation

Fc Block 10 mins room temperature

Blocking Agent

TruStain FcX blocking solution (BioLegend)

Secondary Incubation

2µl of antibody on 1 million cells, 30 mins on ice in the dark (antibody), wash three times afterwards with 2ml of FACS buffer

Tertiary Incubation

None

Detection

Flow Cytometry

Results Summary

Good antibody, specific staining compared to isotype, also reproducible staining of small subpopulations in our panel.

Additional Notes

None

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Summary

The Good

Quick, reliable staining, good fluorescence

The Bad

BV421 conjugate rather expensive, same clone available with different conjugations

The Bottom Line

Easy to use, reliable, quick staining, no fixation etc. required. Because of BV421 conjugation it is rather expensive, but for multiple staining it is very useful

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