Thrombin

October 19, 2015

Texas Tech University Health Science Center
Internal Medicine
Postdoctoral Researcher

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Company:

Sigma-Aldrich

Product Name:

Thrombin from Human Plasma

Catalog Number:

T8885

Thrombin is a serine protease which plays a major role in blood clotting and it is also one of the potent mitogens for major cell types.

Experimental Design and Results Summary

Application

Cell culture

Starting Material

Human plasma

Protocol Overview

Thrombin is widely used as a strong agonist in cell culture studies and other applications. It is an enzyme which will cleave at the recombinant fusion proteins at the recognition site. It is produced at the site of vascular injury and it plays an important role in blood clotting by converting fibrinogen to fibrin.

Tips

Frequent thawing and freezing should be avoided otherwise the activity of the enzyme will be poor.

Results Summary

Thrombin mediates its effects via G protein coupled receptors. Further activation of these G proteins leads to the stimulation of various phospholipases which in turn causes Ca2+ mobilization and PKC activation. Since thrombin is produced at the site of vascular injury it also palys a major role in the endothelial cell barrier disruption and also in inflammation.

Additional Notes

None

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Summary

The Good

It works great and the results are reliable.

The Bad

None

The Bottom Line

Since thrombin plays a role in endothelial barrier disruption and activation of monocytes and macrophages as well as cell formation, all of which is considered to be the hallmarks of various diseases, it is necessary to study the role of thrombin.

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