Best FLAG Antibody for Immunoblot in Worms

October 27, 2015

Genetics
Washington University
post-doc

Overall

Quality of Results

Ease-of-Optimization

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Company:

Sigma-Aldrich

Product Name:

FLAG M2

Catalog Number:

F1804

Browse Similar Products: FLAG M2 Antibodies

This is the most consumed antibody in our lab. The high specificity of this antibody always gives clean blots.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Two transgenes fused with FLAG

Primary Incubation

1:1000 for 2hour

Blocking Agent

5% milk for 1hour

Secondary Incubation

Anti-mouse-HRP 1:10000 for 1hour

Tertiary Incubation

NA

Detection

ECL from Fisher

Results Summary

Always gives consistent results with low background.

Additional Notes

None

Related Categories

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Summary

The Good

Clean results with minimal rounds of tests

The Bad

None

The Bottom Line

Highly suggested for use in immunoblot

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