BD Biosciences
Purified Mouse Anti-Human HIF-1α
610958
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Browse Similar Products: HIF1a Western blot Antibodies
I wanted to test the efficacy of HIF 1 alpha inhibitors in cells. I artificially induced upregulation of HIF 1 alpha using Cobalt chloride and then added my inhibitor of choice. 6 hours post-incubation I made lysates of the cells in RIPA buffer and loaded 10ug of protein on a precast Biorad Protean mini gel. I then performed a western blot for HIF 1 alpha using the antibody.
Western Blot
Lysates of patient glioblastoma cells [Glioma stem cells]
1:1000 overnight at 4C
5%milk in TBST
1:10000 anti mouse HRP for 1.5 hours at room temperature
NO tertiary incubation needed
Chemiluminescence
The antibody worked exceedingly well and did not require optimization. It did not detect any HIF in the control GSCs, detected a large amount of HIF1 alpha in the GSCs treated with cobalt chloride and did not detect any HIF1 alpha in the inhibitor treated cells showing the high specificity of the antibody for the protein
None
No optimization needed. Virtually no background.
None that I detected
Great antibody for western blots