DNA Extraction

University of Miami
Cell Biology
RA3

Overall

Quality of Results

Ease-of-Optimization

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Company:

Omega Biotek

Product Name:

EZNA Plasmid DNA mini kit

Catalog Number:

D6943-00

We constantly have to sequence plasmid DNA in our lab. Before going to a maxi prep we always do a mini prep to send it for sequencing and confirming the sequence is what we want. This great kit is cheap, easy to use, and gives great results.

Experimental Design and Results Summary

Application

DNA extraction

Starting Material

None

Protocol Overview

Grow a 2 ml bacterial culture, spin it down, lyse the pellet, then put on the silica membrane on the column and in 10 mins you have your DNA

Tips

Use Dnase free tips and Dnase free water

Results Summary

Easy to use, easy to optimize

Additional Notes

None

Related Categories

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Summary

The Good

Cheap and fast to do

The Bad

None

The Bottom Line

Highly recommended to get small amount of plasmid DNA to send for sequencing before spending money on a bigger prep

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