Good Isotype Control for APC Flow Antibody

ORTHOPAEDIC SURGERY
RUSH UNIVERSITY MEDICAL CENTER
POSTDOC

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

APC Mouse IgG2a κ Isotype Control

Catalog Number:

400222

Info: View Product View Product Specs

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This isotype control was used for the isotype control of APC conjugated flow antibody. It worked well with double staining with PE color too.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

THP cell line culture

Primary Incubation

NA

Blocking Agent

Human IgG

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Flow cytometry

Results Summary

This isotype control works well and generally is very easy to handle. It worked well with double staining of PE and APC.

Additional Notes

None

Image Gallery

Summary

The Good

Easy and clear protocol and handling

The Bad

Need more information on selecting matched antibody

The Bottom Line

This isotype control worked well in double staining of PE and APC conjugated isotype control.

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