Good Isotype Control for Flow

October 13, 2015

ORTHOPAEDIC SURGERY
RUSH UNIVERSITY MEDICAL CENTER
POSTDOC

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

PE Rat IgG2a κ Isotype Control

Catalog Number:

400507

Info: View Product View Product Specs

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This isotype control was ordered and applied as the isotype control for PE-conjugated flow antibody. It is easy to handle and results were satisfying.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

THP cell line culture

Primary Incubation

NA

Blocking Agent

Human IgG

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Flow cytometry

Results Summary

This isotype was used together with a PE-conjugated flow antibody and it worked well.

Additional Notes

None

Image Gallery

Summary

The Good

Easy to use and tube color matched with corresponding antibody

The Bad

Need more instruction on how to select suitable isotype control in the protocol

The Bottom Line

This isotype control is a good isotype control for PE conjugated flow antibody and it is easy to use.

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