Thermo Fisher Scientific (Invitrogen)
Goat anti-Rat IgG (H+L) Secondary Antibody, Alexa Fluor® 594 conjugate
A-11007
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When I stain tissue sections with a rat-derived primary antibody, I use this product to visualize the stain in the AlexaFluor 594 channel.
Immunofluorescence
Pre-fixed frozen mouse small intestine tissue section
1:100 in 1% BSA in PBS overnight at 4 degrees
5% goat serum in 1% BSA in PBS
Goat anti-rat AlexaFluor594 was used at 1:200 in PBS and was incubated on the section at 1 hour at room temperature in the dark
None
Zeiss LSM 700 confocal
The rat anti-mouse CD31 antibody here is visualized well with the goat anti-rat AF594 secondary stain, with minimal background or artifact staining.
Great secondary antibody stain for rat primary antibodies
None to mention
The product stains rat-derived primary antibodies well, allowing visualization of the stain in the AlexaFluor 594 channel.