Thermo Fisher Scientific (Invitrogen)
Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor® 488 conjugate
A-11034
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When a rabbit-derived primary antibody is used to stain an antigen of interest, an anti-rabbit secondary antibody is necessary to visualize the rabbit anti-mouse signal.
Immunofluorescence
Pre-fixed frozen mouse small intestine tissue section
1:100 in 1% BSA in PBS overnight at 4 degrees
5% goat serum in 1% BSA in PBS
Goat anti-rabbit AlexaFluor488 was used at 1:200 in PBS and was incubated on the section at 1 hour at room temperature in the dark
NA
Zeiss LSM 700 confocal
The rabbit anti-mouse Ki67 stain here is visualized using the goat anti-rabbit AF488 antibody. The stain is also very clean with little background and no apparent staining artifacts.
An intestinal tumor is shown in the image, which is why the Ki67 stain is so widespread
Works as a great secondary antibody for rabbit primaries
None to mention
The product is great for visualizing rabbit primary antibody stains in the AlexaFluor 488 channel.