AF700 Anti Mouse CD3 in Flow Cytometry for Mouse Tissue

September 30, 2015

Internal Medicine.
Rush University Medical Center
Research Assistant

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Company:

BioLegend

Product Name:

Alexa Fluor® 700 anti-mouse CD3 Antibody- BioLegend

Catalog Number:

100216

Info: View Product View Product Specs

Browse Similar Products: CD3 Antibodies

The aim of this experiment was to detect cytokines in mouse T cells. This antibody is good to distinguish the T cell population.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Splenocytes and blood

Primary Incubation

0.5ul AF700 anti-mouse CD3e in 50ul FACS buffer for 30 min at 4oC.

Blocking Agent

0.5 ul CD16/CD32 per sample for 10 min at 4oC.

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

BD LSRII

Results Summary

We got a good graph using this Ab.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Brighter

The Bad

None

The Bottom Line

This antibody is good for multi-color panel.

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