Immune Tech
gp140(mac239)(SIV)
IT-001-140p
For characterising antibody responses to SIV mac gp140 following vaccination or natural infection, ELISAs can be carried out to quantify concentrations, antibody isotypes and their avidities. A robust coating antibody with high reproducibility is required however. This protein can be used for such ELISAs.
ELISA
Each vial contains 100 µg purified protein (1 mg/ml) in PBS.
Dilute the protein in 1x PBS to a concentration of 0.5 ug/ml final.
Coat wells with 100ul of 0.5 ug/ml SIV mac gp140
Highly reproducible antibody titres against the protein are obtained, between experiments, between plates and between protein lots.
None
Availability, price, efficacy
A great product for this ELISA use.