Canine Immunophenotyping

Veterinary Diagnostic Laboratory
Kansas State University
RA

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Alexa Fluor® 647 Mouse IgG2a, κ Isotype Ctrl

Catalog Number:

400234

Info: View Product View Product Specs

Browse Similar Products: IgG2a Antibodies

This product was used to perform immunophenotyping on dog samples for diagnosis of lymphoma. This isotope control was selected to serve as a negative control for the antibodies that were labelled with Alexafluor 647 fluorescent dye.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Whole blood

Primary Incubation

1ul of the isotope for 30mins in the dark at 4 degrees.

Blocking Agent

NA

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

BD fortessa

Results Summary

It served as a good isotope control to help distinguish between the negative and positive populations.

Additional Notes

None

Image Gallery

Summary

The Good

Serves as a good isotope.

The Bad

None

The Bottom Line

Good product.

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