Excellent Antibody for pErk1/2 Immunodetection in Mouse Tissue

October 13, 2015

Physiology
University of Tennessee HSC
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Antibody

Catalog Number:

4370

This antibody was tested for detection of phospho- p44/42 MAPK (Erk1/2) in mouse tissue protein lysate. Mouse tissue samples were collected in RIPA buffer, sonicated and protein extract were prepared. Equal amount of proteins were resolved in 10% SDS-PAGE and immunoblotted for pErk1/2 using this Cell Signaling antibody.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse tissue protein lysate

Primary Incubation

1:1000 (5% BSA in TBST), Overnight at 4 degrees Celsius

Blocking Agent

5% Skimmed milk in TBST (2h)

Secondary Incubation

1:4000 (5%skimmed milk in TBST), 1h at RT

Tertiary Incubation

None

Detection

ECL

Results Summary

Got highly specific bright signal for phosphor-p44/42 in mouse tissue protein extract.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Got bright and highly specific signal

The Bad

None

The Bottom Line

Best antibody I have ever seen. I recommend to all.

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