Cell Signaling Technology
EGFR antibody
4267S
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Our research lab is focused on understanding the role of oncogenes, such as EGFR, in the metabolism, signaling pathways, growth and metastasis of human cancers including prostate, breast, colon and lung. We were testing this antibody to stain EGFR on cancer cells through immunocytocytochemistry.
Immunofluorescence
Cancer cells
1:200 in 2.5% goat serum, overnight at 4 C
2.5% goat serum
1:300, one hour at room temperature
NA
Olympus confocal microscoppy
The antibody worked very well for ICC staining of EGFR compared to the other antibodies we tested.
None
Very good antibody for ICC.
Best antibody for EGFR immunoflurescence experiments.