Western Blot Analysis of Na+/K+-ATPase Alpha 1

Biology
University of Houston
Graduate student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Santa Cruz Biotechnology, Inc.

Product Name:

Na+/K+-ATPase alpha1 (C464.6) Antibody

Catalog Number:

sc-21712

Our research lab is focused on understanding the role of oncogenes, such as EGFR, in the metabolism, signaling pathways, growth and metastasis of human cancers including prostate, breast, colon and lung. We used this product as a plasma membrane marker protein for our subcellular fractionation assays.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Cancer cells

Primary Incubation

1:1000, overnight at 4 C

Blocking Agent

5% milk

Secondary Incubation

1:3000, one hour at room temperature

Tertiary Incubation

NA

Detection

ECL and film

Results Summary

We were able to detect bands with this antibody consistently with little difficulty.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

It can be used as a plasma membrane marker

The Bad

None

The Bottom Line

Good antibody for plasma membrane markers

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