Flow Cytometry Antibody Anti-Human CD11c

Microbiology and Immunology
UIC
Postdoctoral Research Associate

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

FITC anti-human CD11b Antibody

Catalog Number:

301330

Info: View Product View Product Specs

Browse Similar Products: CD11b Antibodies

I used this antibody to characterize human bone and monocyte derived dendritic cells. I was looking for a cheaper antibody and I got this one.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human bone marrow derived dendritic cells

Primary Incubation

1:500 in 100uL FACS Buffer, 4 degrees for 1h

Blocking Agent

NA

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Flow Cytometry using BD CyAn instrument

Results Summary

I used this antibody to characterize human bone dendritic cells derived derived by GM-CSF and IL-4 treatment. Staining was weak and it was hard to get two separate distinct populations using this antibody.

Additional Notes

None

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Summary

The Good

Cheap in price

The Bad

Weak staining

The Bottom Line

Cheapest antibody and reduced quality.

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