Electron Microscopy Sciences
Paraformaldehyde Aqueous Solution -16%
15710
Our lab utilizes paraformaldehyde fixation for immunohistochemistry/immunofluorescence and for fixation of cells for flow cytometry. We utilized paraformaldehyde because it is a common fixation reagent.
Immunohistochemistry/Immunofluorescence, Flow Cytometry (just anything requiring general fixation of cells)
Either fresh frozen tissue cells (for IHC/IF) or single cell suspensions (for flow)
The reagent is provided at 16%. We typically dilute the reagent down to 4% in PBS then fix for varying amount of times, depending on the experiment (typically 10 minutes to 2 hours)
Nothing much.
Cells are well fixed using this reagent, and I have never noticed anything odd resulting from fixation using this product.
Picture is a mouse small intestine section fixed with paraformaldehyde and stained with toluidine blue.
Great at fixing cells, and extremely cheap
Nothing to mention.
The product is a great reagent for fixing cells and is reliable and cheap.