Revvity
SteadyLite
6066756
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This product is easy to use, has high sensitivity, a long lasting signal and no odor.
Glow luminescence (reporter gene assay)
Stably or transiently-transfected mammalian cells
The cells were stimulated with a variety of agents to induce reporter gene expression for 3-8 hours and then equal volume of Steadylite Plus reagent was added. Upon a brief but vigorous shaking (approximately 2 min) the plate can be read on any glow luminometer. Engagement time of 1 second is quite sufficient. Signal lasts over 5 hours.
For best consistency the reagent should be at room temperature prior to adding to the cells.
The reagent is very sensitive and provides a strong signal even at low level of luciferase expression. Yet, it provides an excellent reproducibility.
The reagent is suitable for a broad range of cell concentrations and incubation times. With good expression of the reporter gene signal, strong after 2 hours of incubation.
Sensitive, reproducible, odorless, stable.
Not cheap.
I found Steadylite to be best glow luminescence reagent on the market.