DMEM/F12 Media

September 24, 2015

Centre for Cellular & Molecular Biology
Project JRF
Postdoctoral Researcher

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Company:

Gibco (Life technologies)

Product Name:

DMEM/F12 Media

Catalog Number:

11330-032

This media was used to culture glioblastoma stem cells. The media is ready-made, saves, time and provides less chance of contamination.

Experimental Design and Results Summary

Application

To culture Glioblastoma stem cells.

Starting Material

U87 cells

Protocol Overview

DMEM/F12(1x) with EGF(20ng/mL), bFGF(20ng/mL), N2 supplement(1x), B27 supplement(1x), penstrep(1x), with BSA(0.5mg/mL).

Tips

Make the required amount of media as the growth factors are costly.

Results Summary

In the DMEM/F12 media with growth factors, the U87 cells were grown as spheres (Glioblastoma stem cells).

Additional Notes

Nil

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Summary

The Good

Ready to use, minimal contamination and saves time.

The Bad

Less buffering capacity unlike manually prepared media.

The Bottom Line

Recommended for people who do not want to waste time in media preparation and to minimize contamination chances.

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