Phosphatase Inhibitor

U of Miami
Biology
Student

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Company:

Roche

Product Name:

PhosStop Phosphatase Inhibitor Cocktail Tablets

Catalog Number:

04906837001

This cocktail is used to inhibit phosphatases in protein lysates being assessed for phospho-proteins by western blotting. Easy to dissolve tablets make it easy to make the cocktail solution and it is effective in preserving phosphorylation of proteins.

Experimental Design and Results Summary

Application

Western Blot

Starting Material

None

Protocol Overview

Dissolve tablet in water. Add solution to protein lysis buffer and lyse cells.

Tips

Make sure tablet is completely dissolved before using the solution

Results Summary

Western blots using lysates with this phosphatase cocktail that were probed for phosphorylated proteins show phosphorylated protein signal suggesting phosphatase activity is inhibited.

Additional Notes

None

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Summary

The Good

Easy to use

The Bad

None

The Bottom Line

This is a good product to inhibit phosphatases.

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