Li-Cor
Odyssey CLx
9140-01
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We use western blots and in-cell westerns to analyze changes in protein expression or modification in tissues and cells. We have almost completely transitioned from ECL to near-infrared detection because it works so well.
Western blot detection
Rat liver tissue and rat hepatoma cell lines
The Odyssey is used with near-infrared secondary antibodies for western blot detection and so-called in-cell westerns in which cells are permeabilized to take up the antibodies. It's as simple as using a scanner. Lay the blot, gel, plate, or whathaveyou on the glass panel, set the detector intensity, and press scan.
Watch out for non-specific bands if multiplexing antibodies and use the LiCor strip buffer to strip and reprobe.
This is a great system. No more fiddling with ECL reagents and camera exposure times. The infrared signal remains stable for days so you can image when convenient. Sensitivity and linearity equals or betters ECL. Interestingly, some antibodies that didn't work well with ECL are fine with this system. Multiplexing red and green dyes on one blot has been useful, particularly for comparing phosphorylated vs total. The image shows an anti-ubiquitin primary antibody plus a red IR dye-conjugated secondary antibody.
Besides imaging blots, gels (protein/DNA), and cells, this can image small animal and tissue
None
Versatile imager with stable, sensitive, multiplex signal
Instrument is expensive.
This is a must-have instrument if you're running a lot of blots.
Product Name: Odyssey® CLx Infrared Imaging SystemsSupplier Page